ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Partial Purification of Mulberry (Morus rotunbiloba) Peroxidase Using Aqueous Two-Phase Extraction Coupling with Ion-exchange and Gel-filtration Chromatography)

ผู้เขียน:สุพันธุ์นภา เหลืองหิรัญ, Poontariga Harinnasut, นางอมรา ทองปาน, รองศาสตราจารย์, Amonrat Proomboon, ดร.สุนันทา รัตนาโภ, รองศาสตราจารย์

สื่อสิ่งพิมพ์:pdf

Abstract

The third to fifth leaves of mulberry plant were selected for peroxidase extraction due to its high specific activity comparing to other sets of leaf. The initial enzyme isolation included homogenization and extraction of phenolic compound using aqueous two-phase extraction system consisting of 20/8.9 %(w/v) PEG/(NH)2SO4. This system gave a satisfactorily less partition coefficient (0.018) and also less peroxidase volume (34% of total volume). The purification was performed on a DEAE-Cellulose column and double chromatography of Sephadex G-75. The overall result of mulberry peroxidase purification gave 157.4-folds with 43.4% recovery. The native molecular weight of mulberry calculated from the relative fraction of the standard curve from gel filtration was found to be 29 kDa as which agreed with one band of the molecular weight identified by SDS-PAGE.

Article Info

Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 038, Issue 4, Oct 04 - Dec 04, Page 501 - 509 |  PDF |  Page